Anti-Staphylococcus aureus entB/SEB Polyclonal Antibody (JN006014)

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50ug
100ug

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Overview
Images
References
Datasheet

Overview

Catalog No.

JN006014

Description

Anti-Staphylococcus aureus entB/SEB Polyclonal Antibody (JN006014) is a rabbit polyclonal antibody detecting entB in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Staphylococcus aureus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Staphylococcus aureus entB/SEB (Glu28-Asp251).

Target

Enterotoxin type B, entB, SEB

Purification

Purified by antigen affinity column.

Accession

P01552

RRID

Anti-Staphylococcus aureus entB/SEB Polyclonal Antibody (abinScience Cat# JN006014, RRID:AB_3725072)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Enterotoxin type B (entB/SEB) is a ~31 kDa protein. Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules. In turn, this ternary complex activates a large number of T-lymphocytes initiating a systemic release of pro-inflammatory cytokines. Also causes the intoxication staphylococcal food poisoning syndrome.

1. Marrack, P. et al. (1990) The Journal of experimental medicine 171, 455-64. PMID: 2303780

2. Rödström, KE. et al. (2014) Journal of immunology (Baltimore, Md. : 1950) 193, 1998-2004. PMID: 25015819

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Staphylococcus aureus entB/SEB polyclonal antibody at 1ug/mL on recombinant Staphylococcus aureus entB/SEB (Catalog No. JN006012).

References

Datasheet