Anti-Staphylococcus aureus entD/SED Polyclonal Antibody (JN090014)

Datasheet Share product Copy info

Price(USD): $

Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

JN090014

Description

Anti-Staphylococcus aureus entD/SED Polyclonal Antibody (JN090014) is a rabbit polyclonal antibody detecting entD in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Staphylococcus aureus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Staphylococcus aureus entD/SED (Asn26-Tyr113).

Target

Enterotoxin type D, SED, entD

Purification

Purified by antigen affinity column.

Accession

P20723

RRID

Anti-Staphylococcus aureus entD/SED Polyclonal Antibody (abinScience Cat# JN090014, RRID:AB_3725084)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Enterotoxin type D (entD/SED) is a ~29 kDa protein. Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules. In turn, this ternary complex activates a large number of T-lymphocytes initiating a systemic release of pro-inflammatory cytokines. In addition, induces B-cell proliferation and differentiation in the presence of T-cells. Causes also the intoxication staphylococcal food poisoning syndrome.

1. Al-Daccak, R. et al. (1998) Journal of immunology (Baltimore, Md. : 1950) 160, 225-32. PMID: 9551975

2. Domiati-Saad, R. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 156, 3608-20. PMID: 8621894

3. Kérouanton, A. et al. (2007) International journal of food microbiology 115, 369-75. PMID: 17306397

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Staphylococcus aureus entD/SED polyclonal antibody at 1ug/mL on recombinant Staphylococcus aureus entD/SED (Catalog No. JN090012).

References

Datasheet

Anti-Staphylococcus aureus entD/SED Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Staphylococcus aureus entD/SED Polyclonal Antibody (JN090014)

Overview

Images

References

Datasheet

Quick access

Contact Us