Anti-Influenza B virus (Flu B) NP/Nucleoprotein Polyclonal Antibody (VK528034)

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Overview

Catalog No.

VK528034

Description

Anti-Influenza B virus (Flu B) NP/Nucleoprotein Polyclonal Antibody (VK528034) is a rabbit polyclonal antibody detecting Nucleoprotein in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Influenza B virus (strain B/Lee/1940)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Influenza B virus (Flu B) NP/Nucleoprotein (Met1-Tyr560).

Target

Nucleoprotein, Nucleocapsid protein, Protein N, NP

Purification

Purified by antigen affinity column.

Accession

P04665

RRID

Anti-Influenza B virus (Flu B) NP/Nucleoprotein Polyclonal Antibody (abinScience Cat# VK528034, RRID:AB_3725141)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Nucleoprotein (NP) is a ~61 kDa protein. Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins.

Note

For research use only.

Images

References

Datasheet

Anti-Influenza B virus (Flu B) NP/Nucleoprotein Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Influenza B virus (Flu B) NP/Nucleoprotein Polyclonal Antibody (VK528034)

Overview

Images

References

Datasheet

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