Anti-Phospho-PLC-γ (pY783) Antibody (SAA0303) (HB687013)

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100ug
1mg

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Overview
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Datasheet

Overview

Catalog No.

HB687013

Description

Anti-Phospho-PLC-γ (pY783) Antibody (SAA0303) [SAA0303] (HB687013) is a mouse monoclonal antibody detecting PLC-gamma-1 in IHC, IP, WB. Suitable for Human and Mouse.

Highlights

●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

IHC, IP, WB

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

SAA0303

Clonality

Monoclonal

Target

Phosphoinositide phospholipase C-gamma-1, PLC1, PLC-II, Phospholipase C-II, Phospholipase C-gamma-1, PLC-gamma-1, PLC-148, PLCG1, 1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase gamma-1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P19174

RRID

Anti-Phospho-PLC-γ (pY783) Antibody (SAA0303) (abinScience Cat# HB687013, RRID:AB_3725764)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 (p-PLC-γ) is a ~148 kDa protein. Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, EGFR, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration. Guanine nucleotide exchange factor that binds the GTPase DNM1 and catalyzes the dissociation of GDP, allowing a GTP molecule to bind in its place, therefore enhancing DNM1-dependent endocytosis.

1. Wang, Y. et al. (2007) American journal of physiology. Cell physiology 292, C1775-86. PMID: 17229814

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Phospho-PLC-γ (pY783) Antibody (SAA0303)

References

Datasheet

Anti-Phospho-PLC-γ (pY783) Antibody (SAA0303).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Phospho-PLC-γ (pY783) Antibody (SAA0303) (HB687013)

Overview

Images

References

Datasheet

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