Anti-Clostridioides difficile Toxin A/tcdA Nanobody (SAA0993) (JN085013)

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1mg

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Datasheet

Overview

Catalog No.

JN085013

Description

Anti-Clostridioides difficile Toxin A/tcdA Nanobody (SAA0993) [SAA0993] (JN085013) is a alpaca monoclonal antibody detecting Clostridium difficile Toxin in ELISA.

Species reactivity

Clostridioides difficile (Peptoclostridium difficile)

Applications

ELISA, WB

Host species

Alpaca

Isotype

VHH-8His-Cys-tag

Clone ID

SAA0993

Clonality

Monoclonal

Tested applications

Target

Toxin A, 3.4.22.-, Glucosyltransferase TcdA, 2.4.1.-, tcdA, toxA, Clostridium difficile Toxin

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P16154

RRID

Anti-Clostridioides difficile Toxin A/tcdA Nanobody (SAA0993) (abinScience Cat# JN085013, RRID:AB_3726826)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Toxin A (tcdA) is a ~308 kDa protein. Precursor of a cytotoxin that targets and disrupts the colonic epithelium, inducing the host inflammatory and innate immune responses and resulting in diarrhea and pseudomembranous colitis. TcdA and TcdB constitute the main toxins that mediate the pathology of C.difficile infection, an opportunistic pathogen that colonizes the colon when the normal gut microbiome is disrupted. Compared to TcdB, TcdA is less virulent and less important for inducing the host inflammatory and innate immune responses. This form constitutes the precursor of the toxin: it enters into host cells and mediates autoprocessing to release the active toxin (Glucosyltransferase TcdA) into the host cytosol. Targets colonic epithelia by binding to some receptor, and enters host cells via clathrin-mediated endocytosis.

1. Kuehne, SA. et al. (2010) Nature 467, 711-3. PMID: 20844489

2. Lyras, D. et al. (2009) Nature 458, 1176-9. PMID: 19252482

3. Greco, A. et al. (2006) Nature structural & molecular biology 13, 460-1. PMID: 16622409

4. Tucker, KD. et al. (1991) Infection and immunity 59, 73-8. PMID: 1670930

5. Tao, L. et al. (2019) Nature microbiology 4, 1760-1769. PMID: 31160825

6. Tao, L. et al. (2016) Nature 538, 350-355. PMID: 27680706

7. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356

8. Pruitt, RN. et al. (2009) The Journal of biological chemistry 284, 21934-21940. PMID: 19553670

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Clostridioides difficile Toxin A/tcdA Nanobody (SAA0993)
Bioactivity

Detects Clostridioides difficile Toxin A (Catalog No: JN085012) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-Toxin A monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Clostridioides difficil Toxin A (Catalog No: JN085012)