Anti-Human APLN/Apelin Antibody (SAA1754) (HV404013)

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50ug
100ug
1mg

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Datasheet

Overview

Catalog No.

HV404013

Description

Anti-Human APLN/Apelin Antibody (SAA1754) [SAA1754] (HV404013) is a mouse monoclonal antibody detecting Apelin in ELISA. Suitable for Human.

Species reactivity

Human

Applications

ELISA

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

SAA1754

Clonality

Monoclonal

Target

APEL, Apelin, APJ endogenous ligand, APLN

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q9ULZ1

RRID

Anti-Human APLN/Apelin Antibody (SAA1754) (abinScience Cat# HV404013, RRID:AB_3727515)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Apelin (APLN) is a ~8 kDa protein. Peptide hormone that functions as endogenous ligand for the G-protein-coupled apelin receptor (APLNR/APJ), that plays a role in cadiovascular homeostasis. Functions as a balanced agonist activating both G(i) protein pathway and beta-arrestin pathway of APLNR. Downstream G proteins activation, apelin can inhibit cAMP production and activate key intracellular effectors such as ERKs. On the other hand, APLNR activation induces beta-arrestin recruitment to the membrane leading to desensitization and internalization of the receptor. Apelin blunts cardiac hypertrophic induction from APLNR on response to pathological stimuli, but also induces myocardial hypertrophy under normal conditions.

1. Habata, Y. et al. (1999) Biochimica et biophysica acta 1452, 25-35. PMID: 10525157

2. Scimia, MC. et al. (2012) Nature 488, 394-8. PMID: 22810587

3. Yue, Y. et al. (2022) Nature structural & molecular biology 29, 688-697. PMID: 35817871

4. Wang, WW. et al. (2024) Cell 187, 1460-1475.e20. PMID: 38428423

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human APLN/Apelin Antibody (SAA1754)

References

Datasheet

Anti-Human APLN/Apelin Antibody (SAA1754).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human APLN/Apelin Antibody (SAA1754) (HV404013)

Overview

Images

References

Datasheet

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