Anti-Human NGLY1 Antibody (SAA1882) (HA942013)

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100ug
1mg

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Datasheet

Overview

Catalog No.

HA942013

Description

Anti-Human NGLY1 Antibody (SAA1882) [SAA1882] (HA942013) is a mouse monoclonal antibody detecting NGLY1 in ELISA. Suitable for Human.

Species reactivity

Human

Applications

ELISA

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

SAA1882

Clonality

Monoclonal

Target

PNGase, N-glycanase 1, NGLY1, Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase, hPNGase, PNG1, Peptide:N-glycanase

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q96IV0

RRID

Anti-Human NGLY1 Antibody (SAA1882) (abinScience Cat# HA942013, RRID:AB_3727646)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase (NGLY1) is a ~74 kDa protein. Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins.

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human NGLY1 Antibody (SAA1882)

References

Datasheet

Anti-Human NGLY1 Antibody (SAA1882).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human NGLY1 Antibody (SAA1882) (HA942013)

Overview

Images

References

Datasheet

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