Anti-Human CD106/VCAM1 Nanobody (SAA2055) (HB032013)

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Datasheet

Overview

Catalog No.

HB032013

Description

Anti-Human CD106/VCAM1 Nanobody (SAA2055) [SAA2055] (HB032013) is a alpaca monoclonal antibody detecting VCAM1 in ELISA. Suitable for Human.

Species reactivity

Human

Applications

ELISA

Host species

Alpaca

Isotype

VHH-8His-Cys-tag

Clone ID

SAA2055

Clonality

Monoclonal

Target

Vascular cell adhesion protein 1, VCAM1, CD106, INCAM-100, V-CAM 1, VCAM-1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P19320

RRID

Anti-Human CD106/VCAM1 Nanobody (SAA2055) (abinScience Cat# HB032013, RRID:AB_3729049)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Vascular cell adhesion protein 1 (CD106/VCAM1) is a ~81 kDa protein. Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Also serves as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation.

1. Li, Y. et al. (2020) Journal of molecular cell biology 12, 190-201. PMID: 31310649

2. Taooka, Y. et al. (1999) The Journal of cell biology 145, 413-20. PMID: 10209034

3. Marchese, ME. et al. (2012) Biochemistry 51, 8235-46. PMID: 22970700

4. Pinho, S. et al. (2022) Nature cell biology 24, 290-298. PMID: 35210567

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human CD106/VCAM1 Nanobody (SAA2055)

References

Datasheet

Anti-Human CD106/VCAM1 Nanobody (SAA2055).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human CD106/VCAM1 Nanobody (SAA2055) (HB032013)

Overview

Images

References

Datasheet

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