Anti-Human ASF1B IP Recombinant Antibody (SAA0242) (HP847013)

Name: Anti-Human ASF1B IP Recombinant Antibody (SAA0242)
Brand: abinScience
SKU: HP847013
Price: 191 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug
1mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HP847013

Species reactivity

Human

Applications

ELISA, IF, IP

Host species

Mouse

Isotype

IgG2a

Clone ID

SAA0242

Clonality

Monoclonal

Target

ASF1B, hAsf1, hCIA-II, CIA-II, hAsf1b, Histone chaperone ASF1B, Anti-silencing function protein 1 homolog B, CCG1-interacting factor A-II

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q9NVP2

RRID

Anti-Human ASF1B IP Antibody (SAA0242) (abinScience Cat# HP847013, RRID:AB_3729776)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Histone chaperone ASF1B is a ~22 kDa protein. Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Also involved in the nuclear import of the histone H3-H4 dimer together with importin-4 (IPO4): specifically recognizes and binds newly synthesized histones with the monomethylation of H3 'Lys-9' (H3K9me1) and diacetylation at 'Lys-5' and 'Lys-12' of H4 (H4K5K12ac) marks in the cytosol. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for gonad development.

1. Mello, JA. et al. (2002) EMBO reports 3, 329-34. PMID: 11897662

2. Tagami, H. et al. (2004) Cell 116, 51-61. PMID: 14718166

3. Groth, A. et al. (2005) Molecular cell 17, 301-11. PMID: 15664198

4. Tamburini, BA. et al. (2005) Eukaryotic cell 4, 1583-90. PMID: 16151251

5. Alvarez, F. et al. (2011) The Journal of biological chemistry 286, 17714-21. PMID: 21454524

6. Campos, EI. et al. (2015) Molecular cell 60, 697-709. PMID: 26527279

7. Campos, EI. et al. (2010) Nature structural & molecular biology 17, 1343-51. PMID: 20953179

8. Umehara, T. et al. (2003) The Journal of biological chemistry 278, 35660-7. PMID: 12842904

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human ASF1B IP Antibody (SAA0242)

References

Datasheet

Anti-Human ASF1B IP Recombinant Antibody (SAA0242).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ASF1B IP Recombinant Antibody (SAA0242) (HP847013)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us