Anti-Human B3GAT3 Polyclonal Antibody (HF746014)

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Overview
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Datasheet

Overview

Catalog No.

HF746014

Description

Anti-Human B3GAT3 Polyclonal Antibody (HF746014) is a rabbit polyclonal antibody detecting B3GAT3 in ELISA, IHC, WB. Suitable for Cricetulus griseus, Mus musculus, and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Cricetulus griseus, Mus musculus, Homo sapiens

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human B3GAT3 (Leu74-Val335).

Target

B3GAT3

Purification

Purified by antigen affinity column.

Accession

O94766

RRID

Anti-Human B3GAT3 Polyclonal Antibody (abinScience Cat# HF746014, RRID:AB_3730000)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -130°C for twelve months from the date of receipt.

Alternate Names

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3, 2.4.1.135, Beta-1, 3-glucuronyltransferase 3, Glucuronosyltransferase I, GlcAT-I, UDP-GlcUA:Gal beta-1, 3-Gal-R glucuronyltransferase, GlcUAT-I, B3GAT3

Background

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 (B3GAT3) is a ~37 kDa protein. Glycosaminoglycans biosynthesis. Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc.

1. Budde, BS. et al. (2015) Human genetics 134, 691-704. PMID: 25893793

2. Koike, T. et al. (2014) The Journal of biological chemistry 289, 6695-6708. PMID: 24425863

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human B3GAT3 polyclonal antibody at 1ug/mL on recombinant Human B3GAT3 (Catalog No. HF746012).

References

Datasheet

Anti-Human B3GAT3 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human B3GAT3 Polyclonal Antibody (HF746014)

Overview

Images

References

Datasheet

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