Anti-ZEBOV GP/Envelope glycoprotein Neutralization Antibody (KZ52) (VK623053)

Datasheet Share product Copy info

Price(USD): $

Spec:

50ug
100ug
1mg

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

VK623053

Description

Anti-ZEBOV GP/Envelope glycoprotein Neutralization Antibody (KZ52) [KZ52] (VK623053) is a human monoclonal antibody detecting Envelope glycoprotein in ELISA, WB. Suitable for Zaire ebolavirus.

Species reactivity

Zaire ebolavirus

Applications

ELISA, WB

Host species

Human

Isotype

IgG

Clone ID

KZ52

Clonality

Monoclonal

Target

GP, Envelope glycoprotein

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q05320

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Envelope glycoprotein (GP) is a ~74 kDa protein. Trimeric GP1,2 complexes form the virion surface spikes and mediate the viral entry processes, with GP1 acting as the receptor-binding subunit and GP2 as the membrane fusion subunit. At later times of infection, down-regulates the expression of various host cell surface molecules that are essential for immune surveillance and cell adhesion. Down-modulates several integrins including ITGA1, ITGA2, ITGA3, ITGA4, ITGA5, ITGA6, ITGAV and ITGB1. This decrease in cell adhesion molecules may lead to cell detachment, contributing to the disruption of blood vessel integrity and hemorrhages developed during infection (cytotoxicity) (Probable). Interacts with host TLR4 and thereby stimulates the differentiation and activation of monocytes leading to bystander death of T-lymphocytes.

1. Simmons, G. et al. (2002) Journal of virology 76, 2518-28. PMID: 11836430

2. Takada, A. et al. (2000) Virology 278, 20-6. PMID: 11112476

3. Iampietro, M. et al. (2017) PLoS pathogens 13, e1006397. PMID: 28542576

4. Edri, A. et al. (2018) Frontiers in immunology 9, 1428. PMID: 30013549

5. Vande Burgt, NH. et al. (2015) Viruses 7, 5587-602. PMID: 26516900

6. Brinkmann, C. et al. (2016) Journal of virology 90, 11075-11086. PMID: 27707924

7. González-Hernández, M. et al. (2018) Journal of virology 92. PMID: 29669839

8. Rizk, MG. et al. (2017) Journal of virology 91. PMID: 28878074

Note

For research use only.

Images