Anti-HPeV-1 VP3 Polyclonal Antibody (VK085024)

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Datasheet

Overview

Catalog No.

VK085024

Description

Anti-HPeV-1 VP3 Polyclonal Antibody (VK085024) is a rabbit polyclonal antibody detecting Capsid protein VP3 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human parechovirus 1

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant HPeV-1 Genome polyprotein (Leu361-Gln542).

Target

Genome polyprotein, Capsid protein VP0, P1AB, Virion protein 0, Capsid protein VP3, P1C, Virion protein 3, Capsid protein VP1, P1D, Virion protein 1, Protein 2A H-NC, P2A, Protein 2A, Protein 2B, P2B, Protein 2C, P2C, 3.6.1.15, Protein 3A, P3A, Viral protein genome-linked, VPg, Protein 3B, P3B, Protease 3C, P3C, 3.4.22.28, Picornain 3C, RNA-directed RNA polymerase 3D-POL, P3D-POL, 2.7.7.48

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q66578

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Genome polyprotein (VP3) is a ~245 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry together with capsid proteins VP1 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid proteins interact with host alpha-V/beta-3 integrin heterodimer to provide virion attachment target cell. This attachment induces virion internalization predominantly through clathrin-mediated endocytosis. Binds packaging signals present in the viral RNA.

1. Joki-Korpela, P. et al. (2001) Journal of virology 75, 1958-67. PMID: 11160695

2. Shakeel, S. et al. (2017) Nature communications 8, 5. PMID: 28232749

Note

For research use only

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References

Datasheet