Anti-PVY NIa-Pro Polyclonal Antibody (VK104024)

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Datasheet

Overview

Catalog No.

VK104024

Description

Anti-PVY NIa-Pro Polyclonal Antibody (VK104024) is a rabbit polyclonal antibody detecting NIa-pro in ELISA, IHC, WB. Suitable for Potato virus Y.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Potato virus Y

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant PVY NIa-Pro (Ala2032-Gln2275).

Target

Genome polyprotein, P1 protease, 3.4.21.-, Leader protease P1, N-terminal protein, P1 proteinase, Helper component proteinase, HC-pro, 3.4.22.45, Protein P3, 6 kDa protein 1, 6K1, Cytoplasmic inclusion protein, CI, 3.6.4.-, 6 kDa protein 2, 6K2, Viral genome-linked protein, VPg, Nuclear inclusion protein A, NI-a, NIa, 3.4.22.44, 49 kDa proteinase, 49 kDa-Pro, NIa-pro, Nuclear inclusion protein B, NI-b, NIb, 2.7.7.48, RNA-directed RNA polymerase, Capsid protein, CP, Coat protein

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q02963, P18247

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Genome polyprotein (NIa-Pro) is a ~347 kDa protein. Required for aphid transmission and also has proteolytic activity. Only cleaves a Gly-Gly dipeptide at its own C-terminus. Interacts with virions and aphid stylets. Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May have RNA-binding activity.

Note

For research use only

Images

References

Datasheet

Anti-PVY NIa-Pro Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PVY NIa-Pro Polyclonal Antibody (VK104024)

Overview

Images

References

Datasheet

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