Anti-PV1 Protease 2A Polyclonal Antibody (VK557024)

Datasheet Share product Copy info

Price(USD): $

Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

VK557024

Description

Anti-PV1 Protease 2A Polyclonal Antibody (VK557024) is a rabbit polyclonal antibody detecting Protease 2A in ELISA, IHC, WB. Suitable for Poliovirus type 1, Poliovirus type 2, and Poliovirus type 3.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Poliovirus type 1, Poliovirus type 2, Poliovirus type 3

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant PV1 Protease 2A (Gly882-Gln1030).

Target

Genome polyprotein, P1, Capsid protein VP0, VP4-VP2, Capsid protein VP4, P1A, Virion protein 4, Capsid protein VP2, P1B, Virion protein 2, Capsid protein VP3, P1C, Virion protein 3, Capsid protein VP1, P1D, Virion protein 1, P2, Protease 2A, P2A, 3.4.22.29, Picornain 2A, Protein 2A, Protein 2B, P2B, Protein 2C, P2C, 3.6.1.15, P3, Protein 3AB, Protein 3A, P3A, Viral protein genome-linked, VPg, Protein 3B, P3B, Protein 3CD, 3.4.22.28, Protease 3C, 3.4.22.28, Picornain 3C, P3C, RNA-directed RNA polymerase, RdRp, 2.7.7.48, 3D polymerase, 3Dpol, Protein 3D, 3D

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P06210, P23069, P03300, P06209, P03301, P03302

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Genome polyprotein (Protease 2A) is a ~245 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1 interacts with host cell receptor PVR to provide virion attachment to target host cells. This attachment induces virion internalization predominantly through clathrin- and caveolin-independent endocytosis in Hela cells and through caveolin-mediated endocytosis in brain microvascular endothelial cells.

Note

For research use only

Images

References

Datasheet

Anti-PV1 Protease 2A Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PV1 Protease 2A Polyclonal Antibody (VK557024)

Overview

Images

References

Datasheet

Quick access

Contact Us