Anti-ASFV NP419L Polyclonal Antibody (VK110024)

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50ug
100ug

Number:

Overview
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Datasheet

Overview

Catalog No.

VK110024

Description

Anti-ASFV NP419L Polyclonal Antibody (VK110024) is a rabbit polyclonal antibody detecting ASFV NP419L in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

African swine fever virus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant ASFV NP419L (Met1-Ile419).

Target

DNA ligase, EC:6.5.1.1, Polydeoxyribonucleotide synthase [ATP], LIG

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P35970, P0C990, P0C991, P26813, P0C992

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA ligase (NP419L) is a ~48 kDa protein. Very low-fidelity DNA ligase that seals nicks in double-stranded DNA during DNA repair. Together with the viral repair DNA polymerase X, fills the single nucleotide gaps generated by the AP endonuclease. It is not essential for viral replication and recombination. Displays a very low adenylation activity towards DNA with 3'-dideoxy- or 3'-amino-terminated nicks compared to regular nick DNA.

1. Lamarche, BJ. et al. (2005) Biochemistry 44, 8408-17. PMID: 15938630

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-LIG polyclonal antibody at 1ug/mL on various samples.
Lane 1: ATG21816 100ng

References

Datasheet

Anti-ASFV NP419L Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ASFV NP419L Polyclonal Antibody (VK110024)

Overview

Images

References

Datasheet

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