Anti-Human EGFR/ERBB1/HER1 Antibody (SAA2538) (HF004113)

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Datasheet

Overview

Catalog No.

HF004113

Description

Anti-Human EGFR/ERBB1/HER1 Antibody (SAA2538) [SAA2538] (HF004113) is a rabbit monoclonal antibody detecting ERBB1 in ELISA. Suitable for Human.

Species reactivity

Human

Applications

ELISA

Isotype

scFv-His

Clone ID

SAA2538

Clonality

Monoclonal

Target

EC:2.7.10.1, EGFR, ERBB, ERBB1, Epidermal growth factor receptor, HER1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column

Accession

P00533

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Epidermal growth factor receptor (EGFR/ERBB1) is a ~134 kDa protein. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules.

1. Arcaro, A. et al. (2000) Molecular and cellular biology 20, 3817-30. PMID: 10805725

2. Runkle, KB. et al. (2016) Molecular cell 62, 385-396. PMID: 27153536

3. Chen, WS. et al. (1989) Cell 59, 33-43. PMID: 2790960

4. Bu, J. et al. (2022) Acta biochimica et biophysica Sinica 54, 400-408. PMID: 35538033

5. Garrett, TP. et al. (2002) Cell 110, 763-73. PMID: 12297049

6. Kochupurakkal, BS. et al. (2005) The Journal of biological chemistry 280, 8503-12. PMID: 15611079

7. Kim, J. et al. (2007) Cancer research 67, 9229-37. PMID: 17909029

8. Lu, C. et al. (2010) Molecular and cellular biology 30, 5432-43. PMID: 20837704

Note

For research use only

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SDS-PAGE

SDS-PAGE for Anti-Human EGFR/ERBB1/HER1 Antibody (SAA2538)

References

Datasheet

Anti-Human EGFR/ERBB1/HER1 Antibody (SAA2538).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human EGFR/ERBB1/HER1 Antibody (SAA2538) (HF004113)

Overview

Images

References

Datasheet

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