Anti-Mouse CDw210a/IL10RA Antibody (1B1.3A) (MC614013)

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100ug
1mg

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Overview
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Datasheet

Overview

Catalog No.

MC614013

Description

Anti-Mouse CDw210a/IL10RA Antibody (1B1.3A) [1B1.3A] (MC614013) is a rat monoclonal antibody detecting CDw210a in ELISA, FACS, Functional assay, Research in vivo. Suitable for Mouse.

Highlights

●Multi-Application — Validated across multiple applications.

Species reactivity

Mouse

Applications

ELISA, FACS, Functional assay, Research in vivo

Host species

Rat

Isotype

IgG1, kappa

Clone ID

1B1.3A

Clonality

Monoclonal

Target

CD210, CDw210a, IL-10 receptor subunit alpha, IL-10R subunit 1, IL-10R subunit alpha, IL-10R1, IL-10RA, IL10R, IL10RA, Interleukin-10 receptor subunit 1, Interleukin-10 receptor subunit alpha

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q61727

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Interleukin-10 receptor subunit alpha (CDw210a/IL10RA) is a ~64 kDa protein. Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes.

1. Weber-Nordt, RM. et al. (1996) The Journal of biological chemistry 271, 27954-61. PMID: 8910398

Note

For research use only

Images

References

Datasheet

Anti-Mouse CDw210a/IL10RA Antibody (1B1.3A).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse CDw210a/IL10RA Antibody (1B1.3A) (MC614013)

Overview

Images

References

Datasheet

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