Anti-VEEV Spike glycoprotein E2 Polyclonal Antibody (VK039024)

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Datasheet

Overview

Catalog No.

VK039024

Description

Anti-VEEV Spike glycoprotein E2 Polyclonal Antibody (VK039024) is a rabbit polyclonal antibody detecting Spike glycoprotein E2 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Venezuelan equine encephalitis virus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant VEEV Structural polyprotein (Ser335-Pro679).

Target

6K protein, Assembly protein E3, C, Capsid protein, Coat protein, E1 envelope glycoprotein, E2 envelope glycoprotein, EC:3.4.21.90, Precursor of protein E3/E2, Spike glycoprotein E1, Spike glycoprotein E2, Structural polyprotein, p130, p62, pE2

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P09592

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Structural polyprotein (E2) is a ~138 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

1. Garmashova, N. et al. (2007) Journal of virology 81, 2472-84. PMID: 17108023

2. Garmashova, N. et al. (2007) Journal of virology 81, 13552-65. PMID: 17913819

3. Atasheva, S. et al. (2010) Journal of virology 84, 4158-71. PMID: 20147401

4. Carey, BD. et al. (2018) Journal of virology 92. PMID: 29769351

Note

For research use only

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