Research Grade ombetoxabart (JN876026)

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Spec:

100ug
1mg

Number:

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Datasheet

Overview

Catalog No.

JN876026

Description

Research Grade ombetoxabart (JN876026) is a human monoclonal antibody. Suitable for Clostridioides difficile.

Species reactivity

Clostridioides difficile

Applications

ELISA, Bioactivity: FACS, Functional assay, Research in vivo

Host species

Human

Isotype

IgG1, kappa

Clonality

Monoclonal

Endotoxin level

< 10 EU/mg

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P18177

Form

Liquid

Storage buffer

0.01 M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -101°C long term.

Background

Toxin B (tcdB) is a ~269 kDa protein. Precursor of a cytotoxin that targets and disrupts the colonic epithelium, inducing the host inflammatory and innate immune responses and resulting in diarrhea and pseudomembranous colitis. TcdB constitutes the main toxin that mediates the pathology of C.difficile infection, an opportunistic pathogen that colonizes the colon when the normal gut microbiome is disrupted. Compared to TcdA, TcdB is more virulent and more important for inducing the host inflammatory and innate immune responses. This form constitutes the precursor of the toxin: it enters into host cells and mediates autoprocessing to release the active toxin (Glucosyltransferase TcdB) into the host cytosol. Targets colonic epithelia by binding to the frizzled receptors FZD1, FZD2 and FZD7, and enters host cells via clathrin-mediated endocytosis.

1. Kuehne, SA. et al. (2010) Nature 467, 711-3. PMID: 20844489

2. Xu, H. et al. (2014) Nature 513, 237-41. PMID: 24919149

3. Lyras, D. et al. (2009) Nature 458, 1176-9. PMID: 19252482

4. Qa'Dan, M. et al. (2000) Infection and immunity 68, 2470-4. PMID: 10768933

5. Barth, H. et al. (2001) The Journal of biological chemistry 276, 10670-6. PMID: 11152463

6. Pfeifer, G. et al. (2003) The Journal of biological chemistry 278, 44535-41. PMID: 12941936

7. Reineke, J. et al. (2007) Nature 446, 415-9. PMID: 17334356

9. Tao, L. et al. (2016) Nature 538, 350-355. PMID: 27680706

Note

For research use only.

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