Anti-RUBV Spike Glycoprotein E2 Polyclonal Antibody (VK728014)

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Overview
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Datasheet

Overview

Catalog No.

VK728014

Description

Anti-RUBV Spike Glycoprotein E2 Polyclonal Antibody (VK728014) is a rabbit polyclonal antibody detecting Spike glycoprotein E2. Suitable for Rubella virus.

Species reactivity

Rubella virus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant RUBV Spike Glycoprotein E2 (His376-Ser520).

Target

C, Capsid protein, Coat protein, E1 envelope glycoprotein, E2 envelope glycoprotein, Spike glycoprotein E1, Spike glycoprotein E2, Structural polyprotein, p110

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q8VA10

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Structural polyprotein (Spike Glycoprotein E2) is a ~114 kDa protein. Capsid protein interacts with genomic RNA and assembles into icosahedric core particles 65-70 nm in diameter. The resulting nucleocapsid eventually associates with the cytoplasmic domain of E2 at the cell membrane, leading to budding and formation of mature virions from host Golgi membranes. Phosphorylation negatively regulates RNA-binding activity, possibly delaying virion assembly during the viral replication phase. Capsid protein dimerizes and becomes disulfide-linked in the virion. Modulates genomic RNA replication.

Note

For research use only

Images

References

Datasheet

Anti-RUBV Spike Glycoprotein E2 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-RUBV Spike Glycoprotein E2 Polyclonal Antibody (VK728014)

Overview

Images

References

Datasheet

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