Anti-Human TRIM65 Polyclonal Antibody (HD347014)

Name: Anti-Human TRIM65 Polyclonal Antibody
Brand: abinScience
SKU: HD347014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HD347014

Description

Anti-Human TRIM65 Polyclonal Antibody (HD347014) is a rabbit polyclonal antibody detecting TRIM65. Suitable for Human.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TRIM65 (Val319-Ser517).

Target

E3 ubiquitin-protein ligase TRIM65, EC:2.3.2.27, TRIM65, Tripartite motif-containing protein 65

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q6PJ69

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

E3 ubiquitin-protein ligase TRIM65 is a ~57 kDa protein. E3 ubiquitin ligase that plays a role in several processes including innate immnity, autophagy or inflammation. Negatively regulates miRNAs by modulating the ubiquitination and stability of TNRC6A, a protein involved in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs. This ubiquitination results in the suppressed expression of miR-138-5p leading to increased autophagy. Upon enteroviral infection, promotes 'Lys-63'-mediated ubiquitination activation of IFIH1/MDA5 leading to innate signaling cascade. Mechanistically, selectively recognizes MDA5 filaments that occur on dsRNAs.

1. Meng, J. et al. (2017) Cell death & disease 8, e2866. PMID: 28594402

2. Tang, T. et al. (2021) Frontiers in immunology 12, 741839. PMID: 34512673

3. Li, S. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 6970-5. PMID: 24778252

4. Pan, X. et al. (2019) Cell death & disease 10, 429. PMID: 31160576

5. Kato, K. et al. (2021) Molecular cell 81, 599-613.e8. PMID: 33373584

6. Li, Y. et al. (2020) Journal of molecular cell biology 12, 190-201. PMID: 31310649

Note

For research use only

Images

References

Datasheet

Anti-Human TRIM65 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human TRIM65 Polyclonal Antibody (HD347014)

Overview

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References

Datasheet

SDS

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