Anti-Mouse CA4 Polyclonal Antibody (MB731014)

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

MB731014

Description

Anti-Mouse CA4 Polyclonal Antibody (MB731014) is a rabbit polyclonal antibody detecting CA4. Suitable for Mouse.

Species reactivity

Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Mouse CA4 (Glu18-Ser277).

Target

CA-IV, CA4, Carbonate dehydratase IV, Carbonic anhydrase 4, Carbonic anhydrase IV, EC:4.2.1.1

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q64444

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Carbonic anhydrase 4 (CA4) is a ~34 kDa protein. Catalyzes the reversible hydration of carbon dioxide into bicarbonate and protons and thus is essential to maintaining intracellular and extracellular pH. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid.

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-CA3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: K562 cell lysate
Lane 3: A431 cell lysate

References

Datasheet

Anti-Mouse CA4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse CA4 Polyclonal Antibody (MB731014)

Overview

Images

References

Datasheet

SDS

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