Anti-E. coli groES Polyclonal Antibody (JN912014)

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Datasheet

Overview

Catalog No.

JN912014

Description

Anti-E. coli groES Polyclonal Antibody (JN912014) is a rabbit polyclonal antibody detecting groES. Suitable for strain K12.

Species reactivity

Escherichia coli (strain K12)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant E. coli groES (Met1-Ala97).

Target

10 kDa chaperonin, Chaperonin-10, Co-chaperonin GroES, Cpn10, groES, groS, mopB

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P0A6F9

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Co-chaperonin GroES is a ~10 kDa protein. Together with the chaperonin GroEL, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding, probably by preventing aggregation and by entropically destabilizing folding intermediates. GroES binds to the apical surface of the GroEL ring, thereby capping the opening of the GroEL channel. Regulates the ATPase activity of GroEL.

1. Goloubinoff, P. et al. (1989) Nature 342, 884-9. PMID: 10532860

2. Tang, YC. et al. (2006) Cell 125, 903-14. PMID: 16751100

3. Martin, J. et al. (1991) Nature 352, 36-42. PMID: 1676490

4. Tang, YC. et al. (2008) The EMBO journal 27, 1458-68. PMID: 18418386

5. Apetri, AC. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 17351-5. PMID: 18987317

6. Chakraborty, K. et al. (2010) Cell 142, 112-22. PMID: 20603018

7. Gupta, AJ. et al. (2014) Journal of molecular biology 426, 2739-54. PMID: 24816391

8. Kusukawa, N. et al. (1989) The EMBO journal 8, 3517-21. PMID: 2573517

9. Hemmingsen, SM. et al. (1988) Nature 333, 330-4. PMID: 2897629

Note

For research use only

Images

References

Datasheet

Anti-E. coli groES Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-E. coli groES Polyclonal Antibody (JN912014)

Overview

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References

Datasheet

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