Anti-PIK3C2A Polyclonal Antibody (HT279014)

Name: Anti-PIK3C2A Polyclonal Antibody
Brand: abinScience
SKU: HT279014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HT279014

Description

Anti-PIK3C2A Polyclonal Antibody (HT279014) is a rabbit polyclonal antibody detecting PIK3C2A in ELISA, IHC, WB. Suitable for Human and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PIK3C2A (Phe172-Asn403).

Target

Phosphoinositide 3-kinase-C2-alpha, PIK3C2A, Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha, PtdIns-3-kinase C2 subunit alpha, PI3K-C2-alpha

Purification

Purified by antigen affinity column.

Accession

O00443

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha (PIK3C2A) is a ~190 kDa protein. Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion. Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin-mediated RHOQ activation. Regulates insulin secretion through two different mechanisms: involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion.

1. Tiosano, D. et al. (2019) PLoS genetics 15, e1008088. PMID: 31034465

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PIK3C2A polyclonal antibody at 1ug/mL on recombinant PIK3C2A (Catalog No. HT279012).

References

Datasheet

Anti-PIK3C2A Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PIK3C2A Polyclonal Antibody (HT279014)

Overview

Images

References

Datasheet

SDS

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