Anti-PLK4 Polyclonal Antibody (HT163014)

Name: Anti-PLK4 Polyclonal Antibody
Brand: abinScience
SKU: HT163014
Price: 168 USD
Availability: InStock

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Overview
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Overview

Catalog No.

HT163014

Description

Anti-PLK4 Polyclonal Antibody (HT163014) is a rabbit polyclonal antibody detecting PLK4 in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, Pongo abelii, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PLK4 (Met1-Pro263).

Target

PLK-4, Serine/threonine-protein kinase Sak, Serine/threonine-protein kinase PLK4, STK18, PLK4, Polo-like kinase 4, Serine/threonine-protein kinase 18, SAK

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O00444

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Serine/threonine-protein kinase PLK4 is a ~108 kDa protein. Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors.

1. Tang, CJ. et al. (2011) The EMBO journal 30, 4790-804. PMID: 22020124

2. Denu, RA. et al. (2019) The Journal of biological chemistry 294, 6531-6549. PMID: 30804208

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PLK4 polyclonal antibody at 1ug/mL on recombinant PLK4 (Catalog No. HT163012).

References

Datasheet

Anti-PLK4 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PLK4 Polyclonal Antibody (HT163014)

Overview

Images

References

Datasheet

SDS

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