Anti-APAF1 Polyclonal Antibody (HT398014)

Name: Anti-APAF1 Polyclonal Antibody
Brand: abinScience
SKU: HT398014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HT398014

Description

Anti-APAF1 Polyclonal Antibody (HT398014) is a rabbit polyclonal antibody detecting APAF1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human APAF1 (Ser31-Phe350).

Target

APAF1, APAF-1, Apoptotic protease-activating factor 1, KIAA0413

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O14727

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Apoptotic protease-activating factor 1 (APAF1) is a ~141 kDa protein. Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-APAF1 polyclonal antibody at 1ug/mL on recombinant APAF1 (Catalog No. HT398012).

References

Datasheet

Anti-APAF1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-APAF1 Polyclonal Antibody (HT398014)

Overview

Images

References

Datasheet

SDS

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