Anti-Hypusine antibody (Hpu24) (YP587013)

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Overview
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Datasheet

Overview

Catalog No.

YP587013

Description

Anti-Hypusine antibody (Hpu24) [Hpu24] (YP587013) is a rabbit monoclonal antibody detecting Hypusine in ELISA, IHC, WB. Suitable for General.

Highlights

●Rabbit Monoclonal — High specificity, affinity, and excellent lot-to-lot consistency.
●Multi-Application — Validated across multiple applications.

Species reactivity

General

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clone ID

Hpu24

Clonality

Monoclonal

Target

Hypusine, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, eIF-5A, Rev-binding factor, eIF-4D, EIF5A

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

PubChem: 65396

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Note

For research use only.

Images

Bioactivity

SEC-HPLC detection for Anti-Hypusine antibody (Hpu24).
SDS-PAGE

SDS-PAGE for Anti-Hypusine antibody (Hpu24).
Western blot

Western blot analysis was performed using anti-EIF5A monoclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate
Lane 3: 293T cell lysate
Lane 4: NIH3T3 cell lysate
Lane 5: Mouse pancears lysate
Lane 7: Rat pancears lysate
Western blot

Western blot analysis was performed using anti-Eif5a polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: K562 cell lysate
Lane 4: PC12 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: MCF7 cell lysate
Lane 7: 293T cell lysate