Anti-MAPK13 Polyclonal Antibody (HT360014)

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50ug
100ug

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Datasheet

Overview

Catalog No.

HT360014

Description

Anti-MAPK13 Polyclonal Antibody (HT360014) is a rabbit polyclonal antibody detecting MAPK13 in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, Pan troglodytes, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Bos taurus, Human, Mouse, Pan troglodytes, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MAPK13 (Tyr25-Phe308).

Target

MAPK 13, PRKM13, Mitogen-activated protein kinase 13, MAP kinase 13, Mitogen-activated protein kinase p38 delta, Stress-activated protein kinase 4, SAPK4, MAPK13, MAP kinase p38 delta

Purification

Purified by antigen affinity column.

Accession

O15264

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Mitogen-activated protein kinase 13 (MAPK13) is a ~42 kDa protein. Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as pro-inflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK13 is one of the less studied p38 MAPK isoforms. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MAPK13 polyclonal antibody at 1ug/mL on recombinant MAPK13 (Catalog No. HT360012).

References

Datasheet

Anti-MAPK13 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-MAPK13 Polyclonal Antibody (HT360014)

Overview

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References

Datasheet

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