Anti-PGK1 Polyclonal Antibody (HF674014)

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50ug
100ug

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Datasheet

Overview

Catalog No.

HF674014

Description

Anti-PGK1 Polyclonal Antibody (HF674014) is a rabbit polyclonal antibody detecting PGK1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Cricetulus griseus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat, Bos taurus, Cricetulus griseus, Equus caballus, Chicken, Macaca fascicularis, Notamacropus eugenii, Pan troglodytes, Pongo abelii, Pig

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PGK1 (Ser2-Ile417).

Target

PRP 2, Primer recognition protein 2, PGKA, PGK1, Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein

Purification

Purified by antigen affinity column.

Accession

P00558

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Phosphoglycerate kinase 1 (PGK1) is a ~44 kDa protein. Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines. In addition to its role as a glycolytic enzyme, it seems that PGK1 acts as a polymerase alpha cofactor protein (primer recognition protein). Acts as a protein kinase when localized to the mitochondrion where it phosphorylates pyruvate dehydrogenase kinase PDK1 to inhibit pyruvate dehydrogenase complex activity and suppress the formation of acetyl-coenzyme A from pyruvate, and consequently inhibit oxidative phosphorylation and promote glycolysis. May play a role in sperm motility.

1. Bollong, MJ. et al. (2018) Nature 562, 600-604. PMID: 30323285

2. Fujii, H. et al. (1980) The Journal of biological chemistry 255, 6421-3. PMID: 7391028

3. Gondeau, C. et al. (2008) Nucleic acids research 36, 3620-9. PMID: 18463139

4. Jindal, HK. et al. (1990) The Journal of biological chemistry 265, 6540-3. PMID: 2324090

5. Li, X. et al. (2016) Molecular cell 61, 705-719. PMID: 26942675

6. Chen, Z. et al. (2023) Nature communications 14, 1121. PMID: 36849569

7. Liu, XX. et al. (2016) Human reproduction (Oxford, England) 31, 273-9. PMID: 26677959

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PGK1 polyclonal antibody at 1ug/mL on recombinant PGK1 (Catalog No. HF674012).

References

Datasheet

Anti-PGK1 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PGK1 Polyclonal Antibody (HF674014)

Overview

Images

References

Datasheet

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