Anti-HMGCR Polyclonal Antibody (HY041014)

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50ug
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Overview
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Datasheet

Overview

Catalog No.

HY041014

Description

Anti-HMGCR Polyclonal Antibody (HY041014) is a rabbit polyclonal antibody detecting HMGCR in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Cricetulus griseus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat, Bos taurus, Cricetulus griseus, Mesocricetus auratus, Oryctolagus cuniculus, Pongo abelii, Pig

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HMGCR (Glu441-Asn875).

Target

3-hydroxy-3-methylglutaryl-coenzyme A reductase, HMGCR, HMG-CoA reductase

Purification

Purified by antigen affinity column.

Accession

P04035

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) is a ~97 kDa protein. Catalyzes the conversion of (3S)-hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonic acid, the rate-limiting step in the synthesis of cholesterol and other isoprenoids, thus plays a critical role in cellular cholesterol homeostasis. HMGCR is the main target of statins, a class of cholesterol-lowering drugs.

1. Cuccioloni, M. et al. (2011) Journal of lipid research 52, 897-907. PMID: 21357570

2. Luskey, KL. et al. (1985) The Journal of biological chemistry 260, 10271-7. PMID: 2991281

3. Yogev, Y. et al. (2023) Proceedings of the National Academy of Sciences of the United States of America 120, e2217831120. PMID: 36745799

4. Brown, MS. et al. (1980) Journal of lipid research 21, 505-17. PMID: 6995544

5. Istvan, ES. et al. (2001) Science (New York, N.Y.) 292, 1160-4. PMID: 11349148

6. Sarver, RW. et al. (2008) Journal of medicinal chemistry 51, 3804-13. PMID: 18540668

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-HMGCR polyclonal antibody at 1ug/mL on recombinant HMGCR (Catalog No. HY041012).

References

Datasheet

Anti-HMGCR Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-HMGCR Polyclonal Antibody (HY041014)

Overview

Images

References

Datasheet

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