Anti-P4HB Polyclonal Antibody (HY517014)

Name: Anti-P4HB Polyclonal Antibody
Brand: abinScience
SKU: HY517014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HY517014

Description

Anti-P4HB Polyclonal Antibody (HY517014) is a rabbit polyclonal antibody detecting P4HB in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Cricetulus griseus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat, Bos taurus, Cricetulus griseus, Chicken, Macaca fuscata fuscata, Oryctolagus cuniculus, Pongo abelii

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human P4HB (Phe216-Gly465).

Target

Protein disulfide-isomerase, Cellular thyroid hormone-binding protein, PDI, p55, ERBA2L, PO4DB, Prolyl 4-hydroxylase subunit beta, P4HB, PDIA1

Purification

Purified by antigen affinity column.

Accession

P07237

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Protein disulfide-isomerase (P4HB) is a ~57 kDa protein. This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations and following phosphorylation by FAM20C, functions as a chaperone that inhibits aggregation of misfolded proteins.

1. Yu, J. et al. (2020) The EMBO journal 39, e103841. PMID: 32149426

2. Bi, S. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 10650-5. PMID: 21670307

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-P4HB polyclonal antibody at 1ug/mL on recombinant P4HB (Catalog No. HY517012).

References

Datasheet

Anti-P4HB Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-P4HB Polyclonal Antibody (HY517014)

Overview

Images

References

Datasheet

SDS

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