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Anti-APRT Polyclonal Antibody (HY468014)

Anti-APRT Polyclonal Antibody
Anti-APRT Polyclonal Antibody
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Spec:
  • 50ug
  • 100ug
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  • Datasheet

Overview
Catalog No.HY468014
Description
Anti-APRT Polyclonal Antibody (HY468014) is a rabbit polyclonal antibody detecting APRT in ELISA, IHC, WB. Suitable for Bovine, Cricetulus griseus, Human, and Rat.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityBos taurus, Cricetulus griseus, Dipodillus campestris, Human, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human APRT (Met1-Glu180).
Target APRT, Adenine phosphoribosyltransferase
Purification Purified by antigen affinity column.
Accession P07741
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Adenine phosphoribosyltransferase (APRT) is a ~19 kDa protein. Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.

Note For research use only.
Images
  • Anti-APRT Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-APRT polyclonal antibody at 1ug/mL on recombinant APRT (Catalog No. HY468012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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