Anti-NR3C2 Polyclonal Antibody (HY243014)

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50ug
100ug

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Datasheet

Overview

Catalog No.

HY243014

Description

Anti-NR3C2 Polyclonal Antibody (HY243014) is a rabbit polyclonal antibody detecting NR3C2 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Aotus nancymaae, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat, Aotus nancymaae, Chicken, Ovis aries, Saimiri boliviensis boliviensis, Saimiri sciureus, Pig, Tupaia belangeri, Xenopus laevis

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NR3C2 (Gly789-Lys984).

Target

NR3C2, MR, Mineralocorticoid receptor, MCR, Nuclear receptor subfamily 3 group C member 2, MLR

Purification

Purified by antigen affinity column.

Accession

P08235

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Mineralocorticoid receptor (NR3C2) is a ~107 kDa protein. Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-NR3C2 polyclonal antibody at 1ug/mL on recombinant NR3C2 (Catalog No. HY243012).

References

Datasheet

Anti-NR3C2 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-NR3C2 Polyclonal Antibody (HY243014)

Overview

Images

References

Datasheet

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