Anti-CD8B Polyclonal Antibody (HY433014)

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HY433014

Description

Anti-CD8B Polyclonal Antibody (HY433014) is a rabbit polyclonal antibody detecting CD8B in ELISA, IHC, WB. Suitable for Human and Pongo pygmaeus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Pongo pygmaeus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD8B (Leu22-Pro170).

Target

CD8B1, T-cell surface glycoprotein CD8 beta chain, CD8B, CD8b

Purification

Purified by antigen affinity column.

Accession

P10966

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

T-cell surface glycoprotein CD8 beta chain (CD8B) is a ~23 kDa protein. Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD8B polyclonal antibody at 1ug/mL on recombinant CD8B (Catalog No. HY433012).

References

Datasheet

Anti-CD8B Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CD8B Polyclonal Antibody (HY433014)

Overview

Images

References

Datasheet

SDS

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