Anti-CYP11B1 Polyclonal Antibody (HB959014)

Datasheet Share product Copy info

Price(USD): $

Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HB959014

Description

Anti-CYP11B1 Polyclonal Antibody (HB959014) is a rabbit polyclonal antibody detecting CYP11B1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Papio hamadryas ursinus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CYP11B1 (Gly134-Asn503).

Target

CYP11B1, Cytochrome P-450c11, Steroid 11-beta-hydroxylase, CYP11B1, Cytochrome P450C11, CYPXIB1, Cytochrome P450 11B1, mitochondrial, S11BH

Purification

Purified by antigen affinity column.

Accession

P15538

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cytochrome P450 11B1, mitochondrial (CYP11B1) is a ~57 kDa protein. A cytochrome P450 monooxygenase involved in the biosynthesis of adrenal corticoids. Catalyzes a variety of reactions that are essential for many species, including detoxification, defense, and the formation of endogenous chemicals like steroid hormones. Steroid 11beta, 18- and 19-hydroxylase with preferred regioselectivity at 11beta, then 18, and lastly 19. Catalyzes the hydroxylation of 11-deoxycortisol and 11-deoxycorticosterone (21-hydroxyprogesterone) at 11beta position, yielding cortisol or corticosterone, respectively, but cannot produce aldosterone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate for hydroxylation and reducing the second into a water molecule.

1. Bureik, M. et al. (2002) Endocrine research 28, 351-5. PMID: 12530636

2. Pascoe, L. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 8327-31. PMID: 1518866

3. Curnow, KM. et al. (1991) Molecular endocrinology (Baltimore, Md.) 5, 1513-22. PMID: 1775135

4. Zöllner, A. et al. (2008) The FEBS journal 275, 799-810. PMID: 18215163

5. Strushkevich, N. et al. (2013) Molecular endocrinology (Baltimore, Md.) 27, 315-24. PMID: 23322723

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CYP11B1 polyclonal antibody at 1ug/mL on recombinant CYP11B1 (Catalog No. HB959012).

References

Datasheet

Anti-CYP11B1 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CYP11B1 Polyclonal Antibody (HB959014)

Overview

Images

References

Datasheet

Quick access

Contact Us