Anti-CHRNA3 Polyclonal Antibody (HB019014)

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50ug
100ug

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Overview
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Datasheet

Overview

Catalog No.

HB019014

Description

Anti-CHRNA3 Polyclonal Antibody (HB019014) is a rabbit polyclonal antibody detecting CHRNA3 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat, Bos taurus, Chicken

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CHRNA3 (Ser32-Leu240).

Target

NACHRA3, Neuronal acetylcholine receptor subunit alpha-3, CHRNA3

Purification

Purified by antigen affinity column.

Accession

P32297

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Neuronal acetylcholine receptor subunit alpha-3 (CHRNA3) is a ~57 kDa protein. Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators. CHRNA3 forms heteropentameric neuronal acetylcholine receptors with CHRNB2 and CHRNB4, with CHRNA5, and CHRNB3 as accesory subunits. CHRNA3:CHRNB4 being predominant in neurons of the autonomic ganglia, it is known as ganglionic nicotinic receptor.

1. Gharpure, A. et al. (2019) Neuron 104, 501-511.e6. PMID: 31488329

2. Mann, N. et al. (2019) American journal of human genetics 105, 1286-1293. PMID: 31708116

3. Kuryatov, A. et al. (2011) Molecular pharmacology 79, 119-25. PMID: 20881005

4. Wang, F. et al. (1996) The Journal of biological chemistry 271, 17656-65. PMID: 8663494

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CHRNA3 polyclonal antibody at 1ug/mL on recombinant CHRNA3 (Catalog No. HB019012).

References

Datasheet

Anti-CHRNA3 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CHRNA3 Polyclonal Antibody (HB019014)

Overview

Images

References

Datasheet

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