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Anti-MAGEA4 Polyclonal Antibody (HW770014)

Anti-MAGEA4 Polyclonal Antibody
Anti-MAGEA4 Polyclonal Antibody
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Spec:
  • 50ug
  • 100ug
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  • Datasheet

Overview
Catalog No.HW770014
Description
Anti-MAGEA4 Polyclonal Antibody (HW770014) is a rabbit polyclonal antibody detecting MAGEA4 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human MAGEA4 (Met1-Val317).
Target Cancer/testis antigen 1.4, MAGEA4, MAGE4, CT1.4, Melanoma-associated antigen 4, MAGE-X2 antigen, MAGE-41 antigen, MAGE-4 antigen
Purification Purified by antigen affinity column.
Accession P43358
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Melanoma-associated antigen 4 (MAGEA4) is a ~34 kDa protein. Regulates cell proliferation through the inhibition of cell cycle arrest at the G1 phase. Also negatively regulates p53-mediated apoptosis.

1. Bhan, S. et al. (2012) Oncology reports 28, 1498-502. PMID: 22842486
Note For research use only.
Images
  • Anti-MAGEA4 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-MAGEA4 polyclonal antibody at 1ug/mL on recombinant MAGEA4 (Catalog No. HW770012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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