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|---|---|
| Species reactivity | Human | 
| Applications | FCM | 
| Host species | Human | 
| Isotype | IgG2, kappa | 
| Clone ID | 2.13.2# | 
| Clonality | Monoclonal | 
| Target | CD221, Insulin-like growth factor 1 receptor, IGF1R, Insulin-like growth factor I receptor, IGF-I receptor | 
| Endotoxin level | Please contact with the lab for this information. | 
| Purity | >95% as determined by SDS-PAGE. | 
| Purification | Protein A/G purified from cell culture supernatant. | 
| Accession | P08069 | 
| Form | Liquid | 
| Storage buffer | 0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. | 
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | 
| Note | For research use only. | 

SEC-HPLC detection for Anti-Human CD221/IGF1R Antibody (2.13.2#).

Flow-cytometry using anti-human CD221 antibody. Human HeLa cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD221 antibody monoclonal antibody (Catalog # HY353207 , Red Histogram) at a concentration of 20 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human CD221 antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD221 antibody monoclonal antibody (Catalog # HY353207 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
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