Anti-Human CD182/CXCR2 Antibody (3H9), FITC (HB243117)

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Overview

Catalog No.	HB243117
Description	Anti-Human CD182/CXCR2 Antibody (3H9), FITC [3H9] (HB243117) is a mouse monoclonal antibody detecting CD182 in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	3H9
Conjugation	FITC
Clonality	Monoclonal
Target	IL8RB, GRO/MGSA receptor, C-X-C chemokine receptor type 2, CD182, CXCR-2, IL-8R B, CXC-R2, IL-8 receptor type 2, High affinity interleukin-8 receptor B, CDw128b, CXCR2
Endotoxin level	Please contact with the lab for this information.
Accession	P25025
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	C-X-C chemokine receptor type 2 (CD182/CXCR2) is a ~40 kDa protein. Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2. 1. Murphy, PM. et al. (1991) Science (New York, N.Y.) 253, 1280-3. PMID: 1891716 2. Damaj, BB. et al. (1996) The Journal of biological chemistry 271, 12783-9. PMID: 8662698
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow CytoMetry

Flow-cytometry using FITC anti-human CD182 antibody. THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD182 monoclonal antibody (Catalog: HB243117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

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Datasheet

Anti-Human CD182/CXCR2 Antibody (3H9), FITC.pdf

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Anti-Human CD182/CXCR2 Antibody (3H9), FITC (HB243117)

Overview

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Datasheet

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