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Anti-Mouse CD47/MER6 Antibody (A4), APC (MC359137)

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Anti-Mouse CD47/MER6 Antibody (A4), APC

Anti-Mouse CD47/MER6 Antibody (A4), APC

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Datasheet

Overview

Catalog No.	MC359137
Description	Anti-Mouse CD47/MER6 Antibody (A4), APC [A4] (MC359137) is a mouse monoclonal antibody detecting CD47 in FCM. Suitable for Mouse. Highlights ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivity	Mouse
Applications	FCM
Host species	Mouse
Isotype	VHH, mFc
Clone ID	A4
Conjugation	APC
Clonality	Monoclonal
Target	Leukocyte surface antigen CD47, Integrin-associated protein, IAP, CD47
Endotoxin level	Please contact with the lab for this information.
Accession	Q61735
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	Leukocyte surface antigen CD47 (MER6) is a ~33 kDa protein. Adhesive protein that mediates cell-to-cell interactions. Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins. Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation. Plays a role in modulating pulmonary endothelin EDN1 signaling. Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure. CD47 is the therapeutic target of magrolimab (clinical). 1. Bauer, EM. et al. (2010) Cardiovascular research 88, 471-81. PMID: 20610415 2. Kaur, S. et al. (2013) Scientific reports 3, 1673. PMID: 23591719 3. Rogers, NM. et al. (2017) Cardiovascular research 113, 15-29. PMID: 27742621 4. Manna, PP. et al. (2005) The Journal of biological chemistry 280, 29637-44. PMID: 15917238 5. Ghimire, K. et al. (2020) Cells 9. PMID: 32679764 6. Isenberg, JS. et al. (2008) Annals of surgery 247, 180-90. PMID: 18156939 7. Oldenborg, PA. et al. (2000) Science (New York, N.Y.) 288, 2051-4. PMID: 10856220
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

Flow CytoMetry

Flow-cytometry using APC anti-Mouse CD47 antibody. Mouse spleen cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-Mouse CD47 monoclonal antibody (Catalog: MC359137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Mouse CD47/MER6 Antibody (A4), APC.pdf

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Anti-Mouse CD47/MER6 Antibody (A4), APC