Anti-Human TP53/p53 (R175H mutant) Antibody (F2), PerCP (HY039147)

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Catalog No.	HY039147
Description	Anti-Human TP53/p53 (R175H mutant) Antibody (F2), PerCP [F2] (HY039147) is a mouse monoclonal antibody detecting TP53 in ELISA, FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivity	Human
Applications	ELISA, FCM
Host species	Mouse
Isotype	IgG2a, kappa
Clone ID	F2
Conjugation	PerCP
Clonality	Monoclonal
Target	Antigen NY-CO-13, Cellular tumor antigen p53, P53, Tumor suppressor p53, TP53, Phosphoprotein p53
Endotoxin level	Please contact with the lab for this information.
Accession	P04637
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	Cellular tumor antigen p53 (TP53) is a ~43 kDa protein. Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence. Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Negatively regulates cell division by controlling expression of a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. 1. Guo, A. et al. (2000) Nature cell biology 2, 730-6. PMID: 11025664 2. Bergamaschi, D. et al. (2003) Nature genetics 33, 162-7. PMID: 12524540 3. Louria-Hayon, I. et al. (2003) The Journal of biological chemistry 278, 33134-41. PMID: 12810724 4. An, W. et al. (2004) Cell 117, 735-48. PMID: 15186775 5. Ghosh, A. et al. (2004) Molecular and cellular biology 24, 7987-97. PMID: 15340061 6. Zhao, Y. et al. (2007) The Journal of biological chemistry 282, 11969-81. PMID: 17317671 7. Taira, N. et al. (2007) Molecular cell 25, 725-38. PMID: 17349958 8. Allton, K. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 11612-6. PMID: 19556538 9. Huarte, M. et al. (2010) Cell 142, 409-19. PMID: 20673990 10. Wu, L. et al. (2011) The Journal of biological chemistry 286, 2236-44. PMID: 20959462
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Anti-Human TP53/p53 (R175H mutant) Antibody (F2), PerCP.pdf

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Anti-Human TP53/p53 (R175H mutant) Antibody (F2), PerCP (HY039147)

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