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Anti-Human TSHR/LGR3 Antibody (K1-70), PE (HB852227)

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Overview

Catalog No.	HB852227
Description	Anti-Human TSHR/LGR3 Antibody (K1-70), PE [K1-70] (HB852227) is a mouse monoclonal antibody detecting TSHR in ELISA, FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	ELISA, FCM
Host species	Mouse
Isotype	IgG2a, lambda
Clone ID	K1-70
Conjugation	PE
Clonality	Monoclonal
Target	Thyroid-stimulating hormone receptor, TSH-R, TSHR, Thyrotropin receptor, LGR3, RHD18102
Endotoxin level	Please contact with the lab for this information.
Accession	P16473
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	Thyrotropin receptor (TSHR/LGR3) is a ~86 kDa protein. Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin. Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin. TSHR is coupled to G(s) proteins and mediates the activation of adenylate cyclase. This leads to the generation of cyclic adenosine monophosphate (cAMP), which in turn activates protein kinase A (PKA). PKA subsequently phosphorylates downstream targets involved in thyroid hormone biosynthesis and secretion, including thyroid peroxidase (TPO) and the sodium/iodide symporter (NIS). 1. Costagliola, S. et al. (2002) The EMBO journal 21, 504-13. PMID: 11847099 2. Nakabayashi, K. et al. (2002) The Journal of clinical investigation 109, 1445-52. PMID: 12045258 3. Faust, B. et al. (2022) Nature 609, 846-853. PMID: 35940205 4. Duan, J. et al. (2022) Nature 609, 854-859. PMID: 35940204 5. Morgan, SJ. et al. (2016) Thyroid : official journal of the American Thyroid Association 26, 1794-1803. PMID: 27638195
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Datasheet

Anti-Human TSHR/LGR3 Antibody (K1-70), PE.pdf

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Anti-Human TSHR/LGR3 Antibody (K1-70), PE