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Anti-Human HLA-DRB1 Antibody (1D10), PE (HM837227)

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Overview

Catalog No.	HM837227
Description	Anti-Human HLA-DRB1 Antibody (1D10), PE [1D10] (HM837227) is a mouse monoclonal antibody detecting HLA-DRB1 in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1
Clone ID	1D10
Conjugation	PE
Clonality	Monoclonal
Target	HLA class II histocompatibility antigen, DRB1 beta chain, Human leukocyte antigen DRB1, HLA-DRB1
Endotoxin level	Please contact with the lab for this information.
Accession	P01911
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	HLA class II histocompatibility antigen, DRB1 beta chain (HLA-DRB1) is a ~29 kDa protein. A beta chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the alpha chain HLA-DRA, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DRB1-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells. Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes. In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins. 1. Shams, H. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 1966-77. PMID: 15265931 2. Schulze zur Wiesch, J. et al. (2005) Journal of immunology (Baltimore, Md. : 1950) 175, 3603-13. PMID: 16148104 3. Kwok, WW. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 188, 2537-44. PMID: 22327072 4. Muehling, LM. et al. (2016) Journal of immunology (Baltimore, Md. : 1950) 197, 3214-3224. PMID: 27591323 5. Galperin, M. et al. (2018) Science immunology 3. PMID: 29884618 6. Abelin, JG. et al. (2019) Immunity 51, 766-779.e17. PMID: 31495665 7. Topalian, SL. et al. (1996) The Journal of experimental medicine 183, 1965-71. PMID: 8642306 8. Stern, LJ. et al. (1994) Nature 368, 215-21. PMID: 8145819 9. Schmid, D. et al. (2007) Immunity 26, 79-92. PMID: 17182262
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Datasheet

Anti-Human HLA-DRB1 Antibody (1D10), PE.pdf

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Anti-Human HLA-DRB1 Antibody (1D10), PE