Anti-Staphylococcus aureus Alpha-toxin/hly/AT Antibody (2A3.1) (JN998043)

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Datasheet

Overview

Catalog No.

JN998043

Description

Anti-Staphylococcus aureus Alpha-toxin/hly/AT Antibody (2A3.1) [2A3.1] (JN998043) is a mouse monoclonal antibody detecting Staphylococcus aureus in ELISA, Neutralization, SPR.

Highlights

●Multi-Application — Validated across multiple applications.

Species reactivity

Staphylococcus aureus, Staphylococcus aureus (strain NCTC 8325 / PS 47)

Applications

ELISA, Neutralization, SPR

Host species

Mouse

Isotype

IgG2a

Clone ID

2A3.1

Clonality

Monoclonal

Target

hly, hla, Alpha-hemolysin, Alpha-toxin, Alpha-HL, AT, Staphylococcus aureus, Staphylococcus aureus (strain NCTC 8325 / PS 47)

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P09616, Q2G1X0

RRID

Anti-Staphylococcus aureus Alpha-toxin/hly/AT Antibody (2A3.1) (abinScience Cat# JN998043, RRID:AB_3729028)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Alpha-hemolysin (Alpha-toxin/hly) is a ~35 kDa protein. Alpha-toxin binds to the membrane of eukaryotic cells (particularly red blood cells, RBC) forming pores, resulting in hemolysis, with the release of low-molecular weight molecules leading to eventual osmotic RBC lysis. Human RBCs bind much less alpha-toxin than do rabbit RBCs. Heptamer oligomerization and pore formation is required for lytic activity.

1. Walker, B. et al. (1992) The Journal of biological chemistry 267, 10902-9. PMID: 1587866

2. Wilke, GA. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 13473-8. PMID: 20624979

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Staphylococcus aureus Alpha-toxin/hly/AT Antibody (2A3.1)
Western blot

Western blot analysis was performed using anti-a1AT/Serpin A1/SERPINA1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human a1AT/Serpin A1/SERPINA1 (Catalog No: HF638012)
Lane 2: negative control

References

Datasheet

Anti-Staphylococcus aureus Alpha-toxin/hly/AT Antibody (2A3.1).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Staphylococcus aureus Alpha-toxin/hly/AT Antibody (2A3.1) (JN998043)

Overview

Images

References

Datasheet

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