Anti-SARS-CoV-2 RBD Nanobody (H11-D4) (VK565243)

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50ug
100ug
1mg

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Datasheet

Overview

Catalog No.

VK565243

Description

Anti-SARS-CoV-2 RBD Nanobody (H11-D4) [H11-D4] (VK565243) is a alpaca monoclonal antibody detecting RBD in ELISA, Neutralization.

Species reactivity

SARS-CoV-2 (2019-nCoV)

Applications

ELISA, Neutralization

Host species

Alpaca

Isotype

VHH-8His-Cys-tag

Clone ID

H11-D4

Clonality

Monoclonal

Target

Receptor-Binding Domain, RBD, Spike glycoprotein, S glycoprotein, E2, Peplomer protein, Spike protein S1, S

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P0DTC2

RRID

Anti-SARS-CoV-2 RBD Nanobody (H11-D4) (abinScience Cat# VK565243, RRID:AB_3729547)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Spike glycoprotein (RBD) is a ~141 kDa protein. Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. The major receptor is host ACE2. When S2/S2' has been cleaved, binding to the receptor triggers direct fusion at the cell membrane. When S2/S2' has not been cleaved, binding to the receptor results in internalization of the virus by endocytosis using host TFRC and GRM2 and leading to fusion of the virion membrane with the host endosomal membrane. Alternatively, may use NRP1/NRP2 and integrin as entry receptors.

1. Hoffmann, M. et al. (2020) Cell 181, 271-280.e8. PMID: 32142651

2. Walls, AC. et al. (2020) Cell 181, 281-292.e6. PMID: 32155444

3. Cao, W. et al. (2021) Biophysical journal 120, 1011-1019. PMID: 33607086

4. Takeda, M. (2022) Microbiology and immunology 66, 15-23. PMID: 34561887

5. Wrapp, D. et al. (2020) Science (New York, N.Y.) 367, 1260-1263. PMID: 32075877

6. Ou, X. et al. (2020) Nature communications 11, 1620. PMID: 32221306

7. Wang, J. et al. (2021) Cell discovery 7, 119. PMID: 34903715

8. Wang, X. et al. (2023) Journal of virology 97, e0161122. PMID: 36779763

9. Daly, JL. et al. (2020) Science (New York, N.Y.) 370, 861-865. PMID: 33082294

10. Cantuti-Castelvetri, L. et al. (2020) Science (New York, N.Y.) 370, 856-860. PMID: 33082293

Note

For research use only.