Anti-Naja kaouthia Alpha-cobratoxin Nanobody (SAA2343) (ZC081013)

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50ug
100ug
1mg

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Datasheet

Overview

Catalog No.

ZC081013

Description

Anti-Naja kaouthia Alpha-cobratoxin Nanobody (SAA2343) [SAA2343] (ZC081013) is a alpaca monoclonal antibody detecting Alpha-cobratoxin in ELISA, in vivo neutralization. Suitable for Naja kaouthia.

Species reactivity

Naja kaouthia

Applications

ELISA, in vivo neutralization

Host species

Alpaca

Isotype

VHH-His

Clone ID

SAA2343

Clonality

Monoclonal

Target

Alpha-cobratoxin, Alpha-CT, Alpha-CbT, Alpha-Cbtx, Alpha-Ctx, Alpha-elapitoxin-Nk2a, Alpha-EPTX-Nk2a, Long neurotoxin 1, Siamensis 3

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P01391

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Alpha-cobratoxin is a ~7 kDa protein. Monomer: binds with high affinity to muscular (alpha-1-beta-1-gamma-delta/CHRNA1-CHRNB1-CHRNG-CHRND) nAChR (tested on Torpedo californica, Kd=0.2-4.5 nM) and neuronal alpha-7/CHRNA7 nicotinic acetylcholine receptors (Kd=13-105 nM). Also inhibits GABA(A) channels. Heteropentamer targets studied are composed of alpha-1-beta-3-gamma-2 (GABRA1-GABRB3-GABRG2) subunits (IC(50)=236 nM), alpha-1-beta-2-gamma-2 (GABRA1-GABRB2-GABRG2) subunits (IC(50)=469 nM), alpha-2-beta-2-gamma-2 (GABRA2-GABRB2-GABRG2) subunits (IC(50)=485 nM), alpha-5-beta-3-gamma-2 (GABRA5-GABRB3-GABRG2) subunits (IC(50)=635 nM), and alpha-2-beta-3-gamma-2 (GABRA2-GABRB3-GABRG2) subunits (IC(50)=1099 nM) (activated by 10 uM GABA).

1. Osipov, AV. et al. (2008) The Journal of biological chemistry 283, 14571-80. PMID: 18381281

2. Osipov, AV. et al. (2012) The Journal of biological chemistry 287, 6725-34. PMID: 22223648

3. Servent, D. et al. (1997) The Journal of biological chemistry 272, 24279-86. PMID: 9305882

4. Kudryavtsev, DS. et al. (2015) The Journal of biological chemistry 290, 22747-58. PMID: 26221036

Note

For research use only

Images

SDS-PAGE

SDS-PAGE for Anti-Naja kaouthia Alpha-cobratoxin Nanobody (SAA2343)

References

Datasheet

Anti-Naja kaouthia Alpha-cobratoxin Nanobody (SAA2343).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Naja kaouthia Alpha-cobratoxin Nanobody (SAA2343) (ZC081013)

Overview

Images

References

Datasheet

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