Research Grade Ifabotuzumab (HB681016)

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100ug
1mg

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Overview

Catalog No.	HB681016
Description	Ifabotuzumab (HB681016) is a research-grade recombinant antibody targeting HEK. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Humanized
Isotype	IgG1-kappa
Expression system	Mammalian Cells
Clonality	Monoclonal
Target	HEK, Eph-like tyrosine kinase 1, EK4, EPHA3, Tyrosine-protein kinase receptor ETK1, Tyrosine-protein kinase TYRO4, Ephrin type-A receptor 3, hEK4, ETK, TYRO4, ETK1, Human embryo kinase, EPH-like kinase 4
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P29320
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternate Names	Fibatuzumab, KB-004, 1234137-51-9
Background	Ephrin type-A receptor 3 (EPHA3) is a ~110 kDa protein. Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5. Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration. Also activated by EFNA1, inhibiting epithelial-to-mesenchymal transition of cardiac cells and playing a role in heart development. 1. Lawrenson, ID. et al. (2002) Journal of cell science 115, 1059-72. PMID: 11870224 2. Sharfe, N. et al. (2003) Journal of immunology (Baltimore, Md. : 1950) 170, 6024-32. PMID: 12794130
Note	For research use only. Not suitable for clinical or therapeutic use.

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SEC-HPLC

SEC-HPLC detection for Research Grade Ifabotuzumab.
Bioactivity

Detects EPHA3 in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Ifabotuzumab.
Flow Cytometry

Flow-cytometry using anti-human EPHA3 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human EPHA3 monoclonal antibody (Catalog HB681016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Research Grade Ifabotuzumab.pdf

Formula

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Working Solution

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Recombinant Proteins

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Research Grade Ifabotuzumab (HB681016)

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