













| Catalog No. | HY276016 | 
|---|---|
| Species reactivity | Human | 
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo | 
| Host species | Humanized | 
| Isotype | IgG1-kappa | 
| Expression system | Mammalian Cells | 
| Clonality | Monoclonal | 
| Target | Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP | 
| Endotoxin level | Please contact the lab for this information. | 
| Purity | >95% purity as determined by SDS-PAGE. | 
| Purification | Protein A/G purified from cell culture supernatant. | 
| Accession | P02771 | 
| Form | Liquid | 
| Storage buffer | 0.01M PBS, pH 7.4.                                                 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.  | 
                                        
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C. | 
| Note | For research use only. Not suitable for clinical or therapeutic use. | 

SEC-HPLC detection for Research Grade Tacatuzumab.

Detects AFP/Alpha-fetoprotein in indirect ELISAs.

SDS-PAGE for Research Grade Tacatuzumab.

Flow-cytometry using APC anti-human AFP antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human AFP monoclonal antibody (Catalog: HY276016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using PE anti-human AFP antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human AFP monoclonal antibody (Catalog: HY276016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using FITC anti-human AFP antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human AFP monoclonal antibody (Catalog: HY276016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Western blot analysis was performed using anti-AFP/Alpha-fetoprotein  monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human AFP/Alpha-fetoprotein  (Catalog No: HY276011)
Lane 2: negative control




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