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Research Grade Daclizumab (HF996016)

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Spec:
  • 100ug
  • 1mg
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  • Datasheet
Overview
Catalog No.HF996016
Species reactivityHuman
ApplicationsELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host speciesHumanized
IsotypeIgG1-kappa
Expression systemMammalian Cells
ClonalityMonoclonal
TargetIL2-RA, IL-2R subunit alpha, TAC antigen, IL-2-RA, p55, IL2RA, IL-2 receptor subunit alpha, Interleukin-2 receptor subunit alpha, CD25
Endotoxin levelPlease contact the lab for this information.
Purity>95% purity as determined by SDS-PAGE.
PurificationProtein A/G purified from cell culture supernatant.
AccessionP01589
FormLiquid
Storage buffer0.01M PBS, pH 7.4.
Stability and StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternate NamesBIIB019, DACHYP, 152923-56-3
BackgroundDaclizumab (Zenapax) is a humanized, monoclonal antibody that blocks CD25 (α-subunit of the high-affinity interleukin-2 receptor (IL-2R-HA)). Daclizumab (Zenapax) reversibly binds to CD25and prevents the interaction of IL-2 with the IL-2R-HA. Daclizumab (Zenapax) can be used for multiple sclerosis research.
NoteFor research use only. Not suitable for clinical or therapeutic use.
Images
  • SEC-HPLC

    SEC-HPLC detection for Research Grade Daclizumab.

  • Bioactivity

    Detects Human CD25/IL2RA in indirect ELISAs.

  • Flow Cytometry

    Flow-cytometry using anti-human IL2RA antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human IL2RA monoclonal antibody (Catalog HF996016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • SDS-PAGE

    SDS-PAGE for Research Grade Daclizumab.

  • Flow CytoMetry

    Flow-cytometry using APC anti-human CD25 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human CD25 monoclonal antibody (Catalog: HF996016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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