









| Catalog No. | HF996016 |
|---|---|
| Species reactivity | Human |
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
| Host species | Humanized |
| Isotype | IgG1-kappa |
| Expression system | Mammalian Cells |
| Clonality | Monoclonal |
| Target | IL2-RA, IL-2R subunit alpha, TAC antigen, IL-2-RA, p55, IL2RA, IL-2 receptor subunit alpha, Interleukin-2 receptor subunit alpha, CD25 |
| Endotoxin level | Please contact the lab for this information. |
| Purity | >95% purity as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | P01589 |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C. |
| Alternate Names | BIIB019, DACHYP, 152923-56-3 |
| Background | Vopikitug is an IgG1-kappa, anti-IL2RA (interleukin 2 receptor alpha subunit, IL-2RA, TAC, p55, CD25) homo sapiens monoclonal antibody. Vopikitug shows antineoplastic activity. |
| Note | For research use only. Not suitable for clinical or therapeutic use. |

SEC-HPLC detection for Research Grade Daclizumab.

Detects Human CD25/IL2RA in indirect ELISAs.

Flow-cytometry using anti-human IL2RA antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human IL2RA monoclonal antibody (Catalog HF996016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Research Grade Daclizumab.

Flow-cytometry using APC anti-human CD25 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human CD25 monoclonal antibody (Catalog: HF996016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
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