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|---|---|
| Species reactivity | Human | 
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo | 
| Host species | Chimeric | 
| Isotype | IgG1-kappa | 
| Expression system | Mammalian Cells | 
| Clonality | Monoclonal | 
| Target | p185erbB2, HER2, NGL, Tyrosine kinase-type cell surface receptor HER2, NEU, MLN 19, Proto-oncogene Neu, MLN19, ERBB2, Proto-oncogene c-ErbB-2, CD340, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein | 
| Endotoxin level | Please contact the lab for this information. | 
| Purity | >95% purity as determined by SDS-PAGE. | 
| Purification | Protein A/G purified from cell culture supernatant. | 
| Accession | P04626 | 
| Form | Liquid | 
| Storage buffer | 0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. | 
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C. | 
| Alternate Names | MGAH-22, 1350624-75-7 | 
| Note | For research use only. Not suitable for clinical or therapeutic use. | 

SEC-HPLC detection for Research Grade Margetuximab.

Detects CD340/ERBB2/HER2/NEU in indirect ELISAs.

SDS-PAGE for Research Grade Margetuximab.

Flow-cytometry using APC anti-human CD340 antibody. SK-BR-3 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD340 monoclonal antibody (Catalog: HY286046, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using PE anti-human CD340 antibody. SK-BR-3 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD340 monoclonal antibody (Catalog: HY286046, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using FITC anti-human CD340 antibody. SK-BR-3 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD340 monoclonal antibody (Catalog: HY286046, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
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